N-(2-Acetamido)-2-aminoethanesulfonic acid, N-(Carbamoylmethyl)-2-aminoethanesulfonic acid, N-(Carbamoylmethyl)taurine
N-(2-Acetamido)-2-aminoethanesulfonic acid (ACES) is a zwitterionic buffer used in biochemistry and molecular biology research. It is one of the Good′s buffers with a pH range of 6.15 – 8.35. The preferred range of ACES buffer in an aqueous solution is 6.1 – 7.5.
ACES has been used:
as a component of buffered charcoal yeast extract (BYCE) agar for plating bacteria culture and Buffered Yeast Extract (BYE) liquid medium to culture bacteria
to study the impact of the pH shift on the thermal inactivation of bacterial (Bacillus subtills) spores
as a buffer system to study the depolymerization of starch by α-amylase during exposure to hydrostatic pressure.
It is also suitable for use: as buffers for both agarose and polyacrylamide gel electrophoresis; in the isoelectric focusing of proteins; in the analysis of bacterial autolysins in a discontinuous sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) system.
Linear Formula: | H2NCOCH2NHCH2CH2SO3H |
CAS Number: | 7365-82-4 |
Molecular Weight: | 182.2 |
Beilstein: | 2253770 |
EC Number: | 230-908-4 |
MDL number: | MFCD00008030 |
UNSPSC Code: | 12161700 |
PubChem Substance ID: | 24891495 |
NACRES: | NA.25 |
Assay | ≥99.0% (titration) |
form | crystalline powder |
pH | 3.0-4.5 |
useful pH range | 6.1-7.5 |
pKa (25 °C) | 6.8 |
mp | >220 °C (dec.) (lit.) |
solubility | water: 0.523 g/mL, clear, colorless |
application(s) | diagnostic assay manufacturing |
storage temp. | room temp |
SMILES string | NC(=O)CNCCS(O)(=O)=O |
InChI | 1S/C4H10N2O4S/c5-4(7)3-6-1-2-11(8,9)10/h6H,1-3H2,(H2,5,7)(H,8,9,10) |
InChI key | DBXNUXBLKRLWFA-UHFFFAOYSA-N |
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ORID products are tested extensively in-house and supplied worldwide to the Life Sciences industries.
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