Guanidine hydrochloride CAS 50-01-1
Guanidine hydrochloride CAS 50-01-1
Aminoformamidine hydrochloride, Aminomethanamidine hydrochloride, Guanidinium chloride
| Linear Formula: | NH2C(=NH)NH2 · HCl |
| CAS Number: | 50-01-1 |
| Molecular Weight: | 95.53 |
| Beilstein: | 3591990 |
| EC Number: | 200-002-3 |
| MDL number: | MFCD00013026 |
| UNSPSC Code: | 12352107 |
| PubChem Substance ID: | 24895119 |
| NACRES: | NA.31 |
| biological source | synthetic (organic) |
| grade | Molecular Biology |
| Assay | ≥99% |
| form | crystalline powder |
| storage condition | (Tightly closed. Dry. ) |
| technique(s) | RNA extraction: suitable |
| impurities | ≤0.3% water (Karl Fischer) |
| color | white |
| pH | (25 °C, 4.6 – 6/573 g/L) |
| mp | 180-185 °C (lit.) |
| solubility | H2O: 6 M, clear, colorless |
| density | 1.3 g/cm3 (lit.) |
| cation traces | Pb: ≤5 ppm |
| UV absorption | λ: 260 nm Amax: ≤0.10 |
| λ: 290 nm Amax: ≤0.05 | |
| foreign activity | DNase, RNase, none detected |
| SMILES string | Cl[H].NC(N)=N |
| InChI | 1S/CH5N3.ClH/c2-1(3)4;/h(H5,2,3,4);1H |
| InChI key | PJJJBBJSCAKJQF-UHFFFAOYSA-N |
Description:
Guanidine hydrochloride, also known as aminoformamidine hydrochloride, is a versatile compound widely used in molecular biology and biochemical research. Functioning as a potent chaotropic agent, it disrupts protein and nucleic acid structures, making it invaluable for purifying proteins, particularly mRNA. Its denaturing capabilities extend to unfolding proteins, aiding their purification and subsequent refolding, and it can even solubilize denatured insoluble proteins at higher concentrations.
At lower concentrations, guanidine hydrochloride exhibits a unique effect, promoting the refolding of denatured proteins and restoring enzymatic activity. This property is advantageous for protein renaturation studies. In RNA extraction, guanidine hydrochloride acts as a robust denaturant, disrupting cell structures and inactivating RNA enzymes, ensuring the integrity of extracted RNA. In summary, guanidine hydrochloride is a versatile compound with applications in protein purification, nucleic acid isolation, and protein refolding studies, showcasing its multifaceted utility in molecular biology and biochemical research.
Application:
Guanidine hydrochloride has been used:
as a component of the extraction buffer for the extraction of proteoglycans
in extraction during protein fractionation of ATDC5 cell lines
as a chemical additive to study its effective absorbance spectra in structural analysis
to incubate the device chip to release prostate-specific antigen and for regeneration of aptamer
as a component of the 2,4-dinitrophenylhydrazine (DNPH) solution for detecting protein carbonyls by protein carbonylation study
in RNA isolation to dissociate nucleoproteins and inhibit RNase
Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary.
Biochem/physiol Actions:
Guanidine hydrochloride (GuHCl) is a small hydroscopic molecule. It plays a role in inhibiting heat shock protein 104 (Hsp104) adenosine triphosphatase (ATPase) activity in vivo. It is a potent denaturant and inactivator of several enzymes and proteins. GuHCl can inactivate aminoacylase and papain.It is involved in unfolding and inactivation of alkaline phosphatase in Haliotis diversicolor. At higher concentrations, GuHCl is involved in virus inactivation such as Herpes simplex virus 1 (HSV-1).
Features and Benefits:
Highly versatile surfactant for your cell biology and biochemical research
Suitable for sensitive molecular biology applications
Free from Exonuclease, Dnase and Rnase
Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
Application
ORID products are tested extensively in-house and supplied worldwide to the Life Sciences industries.
Related Products
ORID products are tested extensively in-house and supplied worldwide to the Life Sciences industries.
Company Profile
The capabilities of a chemical company that possesses both production and research and development (R&D) capabilities include
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